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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which results in chromosome instability, and so may well contribute to tumorigenesis. This protein was identified to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital part in advertising and marketing mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two extra splice variants were being described but comprehensive nucleotide sequence information just isn't but offered.

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Blocking peptides are peptides that bind exclusively for the focus on antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged by the antibody.

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This guidebook outlines more achievable optimization of mobile and immunoassay parameters to be sure the absolute best final results are...

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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...

Antibodies sure to the blocking peptide no more bind to your epitope on the concentrate on protein. By evaluating the staining from the blocked antibody compared to the antibody on your own, one can see which staining is unique.

Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which lead for the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...

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